Meridian’s universal Inhibitor-Tolerant RT-qPCR Lo-ROX is a 4x formulation ready-to-use quantitative RT-PCR supermix, containing all components (including a passive reference dye) except primers, probes and templates for direct RT-qPCR. It is designed for developing RT-qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various RT and qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.